复方人参皂苷纳米乳的制备及其急性毒性研究

doi:10.11843/j.issn.0366-6964.2014.04.023

Abstract

Abstract:

By using nanotechnology，ginsenoside(GS) and levamisole hydrochloride(LH) were combined with the carrier nanoemulsion to prepare compound GS and LH nanoemulsion(GS-LH-NE).Then its composition and acute toxicity were evaluated.The optimum blank nanoemulsion，which was used to prepare GS-LH-NE，was screened out through pseudo-ternary phase diagram.The enhancement effect of nanoemulsion containing different content of drug on the immunologic function of immunosuppressive mice were studied through splenic lymphocytes proliferation test，and the nanoemulsion with a better immunologic enhancement effect was screened out for ascertaining the composition of GS-LH-NE.The morphology of GS-LH-NE was observed using transmission electron microscope; the particle size was measured using laser particle size analyzer; the gavage acute toxicity of GS-LH-NE was measured using mice.The ascertained composition of GS-LH-NE was Solutol^®HS-15/glycerol/PEG400/IPM/distilled water(with the mass ratio of 25.33:10.14:2.53:6:56)，in which the content of GS and LH were both 30 mg·mL^－1and 30 mg·mL^－1，respectively.GS-LH-NE was a yellow transparent oil-in-water nanoemulsion，which had spherical droplets with the particle size of 23.08 nm and PDI of 0.237，and its gavage LD₅₀ was 402.85 mg·mL^－1.GS-LH-NE is successfully prepared and provides the possibility to develop immunologic enhancer through nanotechnology.

CLC Number:

S859.53

CAO Fa-hao，OU YANG Wu-qing，WANG Yan-ping. Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(4): 666-671.

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Study on the Preparation and Acute Toxicity of Nanoemulsion Containing Ginsenoside and Levamisole Hydrochloride

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